Immunological express-test for springing and barrenness of domestic cattle diagnostics is the complex of rabbit antibodies against chorionic gonadotropin and the dye alizarin.

The principle of diagnostics consists of qualitative finding sexual hormone in cattle urine – chorionic gonadotropin in immunological hormone reaction (antigen) - antibodies against chorionic gonadotropin.

Blood analysis of domestic cattle in different physiological stages: springing (during the whole period) and barrenness testifies that the content of chorionic gonadotropin in blood serum of domestic cattle during the whole springing period varies from 3.30±0.19 up to 8.42±1.36 ME/I, while barrenness animals don’t have such hormone. The maximum concentration of chorionic gonadotropin is in the third month of springing and is 8.42±1.36 ME/I. The investigations of the hormone content of different age and breeds of animals don’t show the differences of the chorionic gonadotropin level in comparative age and breeds aspects. Therefore, qualitive and quantative determination of chorionic gonadotropin may be the basis for early springing diagnostics of domestic cattle and the hormone may be the marker of springing. According to organoleptic properties, physical-chemical and biological indicators preparation should correspond to the following requirements:

The production form of express-test: test-stripes of orange or light-orange color, packed in packages or the liquid of orange color, poured out in sterile tube-droppers with the first opening control with the volume of 5 and 10 ml.

Immunological express-test for springing and barrenness diagnostics of the cattle has sertificates of quality РОСС RU.АВ51.Н01833. Technical specifications for the product “Immunological test for promoting diagnostics at early stages of springing and barrenness of domestic cattle” (technical specifications ТУ 9388-002-87749850-2012), registered at Ulyanovsk Center for Standardization and Metrology.

Immunological express-test for springing and barrenness of domestic cattle diagnostics was worked out in Ulyanovsk State Agricultural Academy by the group of scientists and engineers which has the state “The Leading Scientific School of Ulyanovsk Region” by the results of 2009 in the field of science “Biology, Agricultural Sciences, Technologies of Living Systems”. There are two Doctors of Biology, eight PhD of Biology and Veterinary in Scientific School.

The leader of Scientific School is Vasilyev Dmitry Arkadyevich, Doctor of Biology, Professor, the Member of Russian Academy of Natural Sciences, the head of the Department of Microbiology, Virology, Epizootology and Veterinary-Sanitary Examination of Ulyanovsk State Agricultural Academy, the member of Scientific Council of Ulyanovsk State Agricultural Academy, dissertation councils of Saratov Agricultural University, Ulyanovsk State Agricultural Academy, the group of infectious pathology of Veterinary Medicine Department of Russian Academy of Agricultural Sciences, the member (academician) of Academy of Veterinary Sciences in the direction of Veterinary-Sanitary Examination, honorary degree “Honoured Science and Technology Worker of Ulyanovsk Region”.

The leader of the project of developing and creation of immunological express-test for springing and barrenness of domestic cattle diagnostics is Bogdanov Ilgizar Ismailovich, PhD of Veterinary, associate professor, the head of the Department of Science and Innovation of Ulyanovsk State Agricultural Academy, associate professor of Clinical Diagnostics of Inner Noncontiguous Diseases and Pathology of Animals. He is the author of more than 40 articles, 5 patents, the leader of a number of projects, performed in the framework of Federal Purpose Program, contests of Russian Humanitarian Scientific Fund and Russian Fund of Fundamental Investigations, agency-level programs of Ministry of Agriculture of Russian Federation, the winner of Ulyanovsk region contest “The Best Innovative Project” in the nomination “The Best Innovative Idea”.

The immunological express-test for springing and barrenness of domestic cattle diagnostics has successfully passed numerous testings on technico-economical and consumption properties on the basis of Ulyanovsk State Agricultural Academy, in agricultural farms of Ulyanovsk region.

The main competitive advantages of express-test are:

1. Express-test is oriented to the use of it on the animal breeding farm and private farms without using specialized equipment and without involvement veterinary specialists
2. Biological liquid for investigation and diagnostics is animal’s urine, which is rather easy to get for the diagnostics
3. The procedure of detection of springing and barrenness animals is safe and eliminates the possibility of infection of veterinary specialists with a number of infection diseases (chlamidiosis, brucellosis and so on)
4. Express-test is intended for the early springing diagnostics – the term of springing at which the diagnosis can be done is beginning with the 15^th day after insemination
5. The procedure of diagnostics duration is usually not more than 15 minutes
6. Express-test has a high accuracy and reliability of the performing diagnostics (not less than 98%)
7. Express-test allows to determine positive or negative reaction visually

Yes, the immunological express-test for springing and barrenness of domestic cattle diagnostics can be used for springing diagnostics of horses, sheep, goats, pigs, camels.

According to the investigations, carried out by the team of scientists of Ulyanovsk State Agricultural Academy in the framework of fulfillment of the state contract with the Fund of Assistance of Development of Small Enterprises in scientific-technical field on the subject “The formation of express-test production for springing and barrenness detection of domestic cattle”, blood test of domestic cattle in different physiological stages: springing (during the whole period) and barrenness testifies that the content of chorionic gonadotropin in blood serum of domestic cattle during the whole springing period varies from 3.30±0.19 up to 8.42±1.36 ME/I, while barrenness animals don’t have such hormone. The maximum concentration of chorionic gonadotropin is in the third month of springing and is 8.42±1.36 ME/I. The investigations of the hormone content of different age and breeds of animals don’t show the differences of the chorionic gonadotropin level in comparative age and breeds aspects. Therefore, qualitive and quantative determination of chorionic gonadotropin may be the basis for early springing diagnostics of domestic cattle and the hormone may be the marker of springing.

The price of 1 dose of immunological express-test for springing and barrenness of domestic cattle diagnostics is to 150 roubles, depending on the amount of buying tests.

Express-test is transported according to the cargo and luggage transportation rules, according to ГОСТ 17768-90.

Express-test is stored in dark dry place at temperature +2…+8 ⁰C

Our company guaranties that preparation “one-stage immunological express-test for springing and barrenness of domestic cattle diagnostics” corresponds to the requirements of technical conditions on the product “Immunological express-test for springing and barrenness of domestic cattle diagnostics” maintaining storage conditions and applying stated by the given technical conditions by the consumer.

Preparation validity is 12 months. The date of production is considered to be the date of its packing.